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recombinant tnfα standards  (R&D Systems)


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    Structured Review

    R&D Systems recombinant tnfα standards
    Recombinant Tnfα Standards, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+protein+standards/Recombinant+Human+TNF-alpha+Protein/10__3390_slash_immuno6020029-150-35-38
    Average 97 stars, based on 1743 article reviews
    recombinant tnfα standards - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood.
    Article Snippet: Measurements of the soluble biomarkers PD-1, PD-L1, and PD-L2 were performed with a self-developed enzyme-linked immunosorbent assay (ELISA). .. Two matching antibodies and recombinant protein standards were purchased from R&D Systems (Human PD-1 DuoSet® ELISA: DY1086/Human PD-L1 DuoSet® ELISA: DY156/Human PD-L2/B7DC DuoSet® ELISA: DY1224, R&D Systems, Inc., Minneapolis, MN, USA) to create the assay. ..

    Article Title: Acute sleep disruption- and high-fat diet-induced hypothalamic inflammation are not related to glucose tolerance in mice.
    Article Snippet: .. To generate a standard series, lyophilized recombinant protein standards from R&D Systems (401-ML, 406-ML, and 410-ML for IL-1β, IL-6, and TNF-α, respectively) were reconstituted as per manufacturer’s instructions. .. Diluents were generated with cell lysis buffer (BioRad, Hercules, CA, catalog #171–304012).

    Article Title: Quantification of Protein Biomarkers in SonoPODography Fluid Using Single Molecule Arrays for Endometriosis
    Article Snippet: .. Calibrators were prepared by performing serial dilutions of recombinant protein standards (VEGF cat# BT-VEGF, R&D Systems; CA125 cat# DY5609-05, R&D Systems) in Homebrew Detector/Sample Diluent, along with high and low controls. ..

    Article Title: Missing prognostic value of soluble PD-1, PD-L1 and PD-L2 in lung cancer patients undergoing chemotherapy – A CEPAC-TDM biomarker substudy
    Article Snippet: .. Recombinant protein standards, controls and respective antibodies were bought from R&D Systems (Human PD-1 DuoSet® ELISA: DY1086/ Human PD-L1 DuoSet® ELISA: DY156/ Human PD-L2/B7-DC DuoSet® ELISA: DY1224, all: R&D Systems, Inc., Minneapolis, USA). ..

    Article Title: Ultrasensitive digital quantification of cytokines and bacteria predicts septic shock outcomes.
    Article Snippet: We used the following consumables: Eppendorf 96-Well twin.tec PCR plates (Eppendorf, #951020362), 0.2-μl thin-walled PCR tubes (Thermo Fisher Scientific, #AB-0620), 0.2-μl thin-walled PCR strips (Thermo Fisher Scientific, #AB-1182), and 1.5-ml microcentrifuge tubes (Ambion, #AM12450). .. The biotinylated antibodies (BAB), recombinant protein standards were from R&D Systems: biotinylated anti-human IL-6 polyclonal goat antibody (#BAF206), biotinylated anti-human TNF-α polyclonal goat antibody (#BAF210), recombinant human (RH) IL-6 (#206-IL-010), RH TNF-α (#210-TA-020), and RH IL-10 (#217- IL-005). .. Chicken plasma was purchased from Sigma (#G2282236).

    Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
    Article Snippet: The absorbance wasmeasured with a Synergy 2 plate reader (BioTek Instruments,Winooski, VT, USA) at 450 nm as the primary wavelength. .. Peritoneal lavage fluid was analyzed for CXCL1 secretion by an enzyme-linked immunosorbent assay using corresponding capture and detection antibodies and recombinant protein standards (CXCL1: MAB453, BAF453, 453-KC-010; R&D Systems), followed by Streptavidin-HRP (DY998, R&D Systems) and TMB Substrate incubation (555214, Becton, Dickinson and Company). ..

    Article Title: Mutants of Type II Heat-Labile Enterotoxin LT-IIa with Altered Ganglioside-Binding Activities and Diminished Toxicity Are Potent Mucosal Adjuvants
    Article Snippet: The levels of IL-4, IL-10, and gamma interferon (IFN- ) in culture supernatants were determined by a Luminex cytokine assay, as follows. .. Capture and detection antibody pairs directed against different noncompeting epitopes of the cytokine and recombinant protein standards for IL-4, IL-10, and IFN- were purchased from R&D Systems (Minneapolis, MN). .. Antibodies were covalently coupled to Multi-Analyte carboxylated microspheres (Luminex Corp., Austin, TX) according to the manufacturer’s directions.

    Article Title: An off-chip platform for on-demand, single-target encapsulation for ultrasensitive biomarker detection.
    Article Snippet: .. Recombinant protein standards (R&D Systems 285-IF) were serially diluted to the desired concentration in PBS buffer. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood.
    Article Snippet: Measurements of the soluble biomarkers PD-1, PD-L1, and PD-L2 were performed with a self-developed enzyme-linked immunosorbent assay (ELISA). .. Two matching antibodies and recombinant protein standards were purchased from R&D Systems (Human PD-1 DuoSet® ELISA: DY1086/Human PD-L1 DuoSet® ELISA: DY156/Human PD-L2/B7DC DuoSet® ELISA: DY1224, R&D Systems, Inc., Minneapolis, MN, USA) to create the assay. ..

    Article Title: Missing prognostic value of soluble PD-1, PD-L1 and PD-L2 in lung cancer patients undergoing chemotherapy – A CEPAC-TDM biomarker substudy
    Article Snippet: .. Recombinant protein standards, controls and respective antibodies were bought from R&D Systems (Human PD-1 DuoSet® ELISA: DY1086/ Human PD-L1 DuoSet® ELISA: DY156/ Human PD-L2/B7-DC DuoSet® ELISA: DY1224, all: R&D Systems, Inc., Minneapolis, USA). ..

    Incubation:

    Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
    Article Snippet: The absorbance wasmeasured with a Synergy 2 plate reader (BioTek Instruments,Winooski, VT, USA) at 450 nm as the primary wavelength. .. Peritoneal lavage fluid was analyzed for CXCL1 secretion by an enzyme-linked immunosorbent assay using corresponding capture and detection antibodies and recombinant protein standards (CXCL1: MAB453, BAF453, 453-KC-010; R&D Systems), followed by Streptavidin-HRP (DY998, R&D Systems) and TMB Substrate incubation (555214, Becton, Dickinson and Company). ..

    Concentration Assay:

    Article Title: An off-chip platform for on-demand, single-target encapsulation for ultrasensitive biomarker detection.
    Article Snippet: .. Recombinant protein standards (R&D Systems 285-IF) were serially diluted to the desired concentration in PBS buffer. ..



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    Increased <t>MMP7</t> in BA patients correlated with the progression of liver fibrosis. Serum and liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Serum MMP7 levels were determined by ELISA. ( B ) Relative MMP7 mRNA levels in livers of BA and non-BA patients. ( C ) Representative images of immunostaining for MMP7 and quantitative analysis of MMP7 staining in liver tissue. Magnification: left panel: ×100; right panel: ×400. ( D ) Representative Masson trichrome staining images of METAVIR fibrosis stages (F1–F4) in BA patients. Scale bar: 400 μm. ( E – G ) Serum MMP7 levels, relative MMP7 mRNA levels and the expression intensity of MMP7 in liver of BA patients with moderate (F0–F2) or advanced liver fibrosis (F3–F4). ( H ) Spearman’s rank correlation analysis of MMP7 expression level with fibrotic marker genes, including COL1A1 , ACTA2 , TGFB1 , and TIMP1 .
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    Increased <t>MMP7</t> in BA patients correlated with the progression of liver fibrosis. Serum and liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Serum MMP7 levels were determined by ELISA. ( B ) Relative MMP7 mRNA levels in livers of BA and non-BA patients. ( C ) Representative images of immunostaining for MMP7 and quantitative analysis of MMP7 staining in liver tissue. Magnification: left panel: ×100; right panel: ×400. ( D ) Representative Masson trichrome staining images of METAVIR fibrosis stages (F1–F4) in BA patients. Scale bar: 400 μm. ( E – G ) Serum MMP7 levels, relative MMP7 mRNA levels and the expression intensity of MMP7 in liver of BA patients with moderate (F0–F2) or advanced liver fibrosis (F3–F4). ( H ) Spearman’s rank correlation analysis of MMP7 expression level with fibrotic marker genes, including COL1A1 , ACTA2 , TGFB1 , and TIMP1 .
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    Increased <t>MMP7</t> in BA patients correlated with the progression of liver fibrosis. Serum and liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Serum MMP7 levels were determined by ELISA. ( B ) Relative MMP7 mRNA levels in livers of BA and non-BA patients. ( C ) Representative images of immunostaining for MMP7 and quantitative analysis of MMP7 staining in liver tissue. Magnification: left panel: ×100; right panel: ×400. ( D ) Representative Masson trichrome staining images of METAVIR fibrosis stages (F1–F4) in BA patients. Scale bar: 400 μm. ( E – G ) Serum MMP7 levels, relative MMP7 mRNA levels and the expression intensity of MMP7 in liver of BA patients with moderate (F0–F2) or advanced liver fibrosis (F3–F4). ( H ) Spearman’s rank correlation analysis of MMP7 expression level with fibrotic marker genes, including COL1A1 , ACTA2 , TGFB1 , and TIMP1 .
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    Image Search Results


    Increased MMP7 in BA patients correlated with the progression of liver fibrosis. Serum and liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Serum MMP7 levels were determined by ELISA. ( B ) Relative MMP7 mRNA levels in livers of BA and non-BA patients. ( C ) Representative images of immunostaining for MMP7 and quantitative analysis of MMP7 staining in liver tissue. Magnification: left panel: ×100; right panel: ×400. ( D ) Representative Masson trichrome staining images of METAVIR fibrosis stages (F1–F4) in BA patients. Scale bar: 400 μm. ( E – G ) Serum MMP7 levels, relative MMP7 mRNA levels and the expression intensity of MMP7 in liver of BA patients with moderate (F0–F2) or advanced liver fibrosis (F3–F4). ( H ) Spearman’s rank correlation analysis of MMP7 expression level with fibrotic marker genes, including COL1A1 , ACTA2 , TGFB1 , and TIMP1 .

    Journal: International Journal of Molecular Sciences

    Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

    doi: 10.3390/ijms27052209

    Figure Lengend Snippet: Increased MMP7 in BA patients correlated with the progression of liver fibrosis. Serum and liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Serum MMP7 levels were determined by ELISA. ( B ) Relative MMP7 mRNA levels in livers of BA and non-BA patients. ( C ) Representative images of immunostaining for MMP7 and quantitative analysis of MMP7 staining in liver tissue. Magnification: left panel: ×100; right panel: ×400. ( D ) Representative Masson trichrome staining images of METAVIR fibrosis stages (F1–F4) in BA patients. Scale bar: 400 μm. ( E – G ) Serum MMP7 levels, relative MMP7 mRNA levels and the expression intensity of MMP7 in liver of BA patients with moderate (F0–F2) or advanced liver fibrosis (F3–F4). ( H ) Spearman’s rank correlation analysis of MMP7 expression level with fibrotic marker genes, including COL1A1 , ACTA2 , TGFB1 , and TIMP1 .

    Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Immunostaining, Staining, Expressing, Marker

    MMP7 gene correlation analysis and pathway enrichment in BA. ( A ) Distribution of correlation coefficients between MMP7 expression and all other genes across two independent BA datasets ( GSE15235 and GSE46960 ). ( B ) GSEA results using gene lists ranked by correlation with MMP7.

    Journal: International Journal of Molecular Sciences

    Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

    doi: 10.3390/ijms27052209

    Figure Lengend Snippet: MMP7 gene correlation analysis and pathway enrichment in BA. ( A ) Distribution of correlation coefficients between MMP7 expression and all other genes across two independent BA datasets ( GSE15235 and GSE46960 ). ( B ) GSEA results using gene lists ranked by correlation with MMP7.

    Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

    Techniques: Expressing

    EMT scores were associated with MMP7, liver fibrosis and survival in BA patients. Liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Representative image of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in liver biopsies samples. Magnification: left panel : ×100; right panel : ×400. ( B ) Percentage of samples according to the intensity and extension of EMT-related protein immunostaining in cholangiocytes from two groups. ( C ) EMT scores of cholangiocytes in non-BA and BA patients. ( D ) EMT scores in two subgroups divided by MMP7 expression intensity in BA patients. ( E ) Correlation analysis of fibrosis stage EMT scores in BA patients. Spearman’s rank correlation.

    Journal: International Journal of Molecular Sciences

    Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

    doi: 10.3390/ijms27052209

    Figure Lengend Snippet: EMT scores were associated with MMP7, liver fibrosis and survival in BA patients. Liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Representative image of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in liver biopsies samples. Magnification: left panel : ×100; right panel : ×400. ( B ) Percentage of samples according to the intensity and extension of EMT-related protein immunostaining in cholangiocytes from two groups. ( C ) EMT scores of cholangiocytes in non-BA and BA patients. ( D ) EMT scores in two subgroups divided by MMP7 expression intensity in BA patients. ( E ) Correlation analysis of fibrosis stage EMT scores in BA patients. Spearman’s rank correlation.

    Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

    Techniques: Immunostaining, Expressing

    MMP7 promoted EMT in an E-cadherin/β-catenin pathway-dependent manner. ( A ) Protein expression of E-cadherin in HIBEpiCs after treatment with different concentrations of MMP7 for 24 h. ( B ) Level of sE-cadherin in the cell culture supernatant of HIBEpiCs after MMP7 treatment. ( C ) Western blot analysis of β-catenin protein in the nucleus or cytoplasm of HIBEpiCs. ( D ) Immunofluorescence staining shows the subcellular localization of β-catenin in HIBEpiCs. Scale bar:50 μm. ( E ) TOP/FOP-Flash luciferase activity of MMP7-treated cells. ( F ) Effect of the MMP7 and ICG-001 on β-catenin downstream target genes expression (including SNAI1 , SNAI2 , MMP7 ) in HIBEpiCs. ( G , H ) Effect of the MMP7 and ICG-001 on mRNA and protein expression of EMT-related markers (including E-cadherin, Vimentin, S100A4) in HIBEpiCs. ( I ) ELISA analysis of serum level of sE-cadherin in patients. ( J ) Spearman’s rank correlation analysis of serum sE-cadherin and serum MMP7 in BA patients. ( K ) Representative images of immunostaining of β-catenin in cholangiocytes of non-BA and BA patients. Magnification: left panel : ×100; right panel : ×400. ( C – H ) MMP7 concentration: 80 ng/mL; ICG-001 concentration: 25 μM. Data are presented as mean ± SD.

    Journal: International Journal of Molecular Sciences

    Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

    doi: 10.3390/ijms27052209

    Figure Lengend Snippet: MMP7 promoted EMT in an E-cadherin/β-catenin pathway-dependent manner. ( A ) Protein expression of E-cadherin in HIBEpiCs after treatment with different concentrations of MMP7 for 24 h. ( B ) Level of sE-cadherin in the cell culture supernatant of HIBEpiCs after MMP7 treatment. ( C ) Western blot analysis of β-catenin protein in the nucleus or cytoplasm of HIBEpiCs. ( D ) Immunofluorescence staining shows the subcellular localization of β-catenin in HIBEpiCs. Scale bar:50 μm. ( E ) TOP/FOP-Flash luciferase activity of MMP7-treated cells. ( F ) Effect of the MMP7 and ICG-001 on β-catenin downstream target genes expression (including SNAI1 , SNAI2 , MMP7 ) in HIBEpiCs. ( G , H ) Effect of the MMP7 and ICG-001 on mRNA and protein expression of EMT-related markers (including E-cadherin, Vimentin, S100A4) in HIBEpiCs. ( I ) ELISA analysis of serum level of sE-cadherin in patients. ( J ) Spearman’s rank correlation analysis of serum sE-cadherin and serum MMP7 in BA patients. ( K ) Representative images of immunostaining of β-catenin in cholangiocytes of non-BA and BA patients. Magnification: left panel : ×100; right panel : ×400. ( C – H ) MMP7 concentration: 80 ng/mL; ICG-001 concentration: 25 μM. Data are presented as mean ± SD.

    Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

    Techniques: Expressing, Cell Culture, Western Blot, Immunofluorescence, Staining, Luciferase, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunostaining, Concentration Assay

    Fibrosis progression in chronic BA mice was accompanied by increased MMP7 expression and EMT. Liver and serum samples of the control group (i.p. saline) and chronic BA group (i.p. RRV+ anti-Ly6G) were collected on days 14, 21, and 42 postnatally ( n = 5~7 mice in each group). ( A ) Representative micrograph of Masson trichrome staining of the liver from control and chronic BA mice. Quantification of collagen staining in the liver of control and chronic BA mice. Magnification: ×40. ( B , C ) Serum level and hepatic mRNA expression of MMP7 in control and chronic BA mice. ( D ) Representative images of immunostaining for MMP7 expression in liver (Days 42). ( E ) Representative images of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in liver (Days 42). ( F ) Serum level of sE-cadherin in mice ( n = 5 mice in each group). ( G ) Representative images of immunostaining β-catenin in cholangiocytes of mice. Magnification: left panel : ×100; right panel : ×400. Data are presented as mean ± SD.

    Journal: International Journal of Molecular Sciences

    Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

    doi: 10.3390/ijms27052209

    Figure Lengend Snippet: Fibrosis progression in chronic BA mice was accompanied by increased MMP7 expression and EMT. Liver and serum samples of the control group (i.p. saline) and chronic BA group (i.p. RRV+ anti-Ly6G) were collected on days 14, 21, and 42 postnatally ( n = 5~7 mice in each group). ( A ) Representative micrograph of Masson trichrome staining of the liver from control and chronic BA mice. Quantification of collagen staining in the liver of control and chronic BA mice. Magnification: ×40. ( B , C ) Serum level and hepatic mRNA expression of MMP7 in control and chronic BA mice. ( D ) Representative images of immunostaining for MMP7 expression in liver (Days 42). ( E ) Representative images of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in liver (Days 42). ( F ) Serum level of sE-cadherin in mice ( n = 5 mice in each group). ( G ) Representative images of immunostaining β-catenin in cholangiocytes of mice. Magnification: left panel : ×100; right panel : ×400. Data are presented as mean ± SD.

    Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

    Techniques: Expressing, Control, Saline, Staining, Immunostaining

    MMP7 blockade attenuated liver fibrosis and EMT procession in chronic BA mice. ( A ) Schematic representation of chronic BA mice injected with anti-MMP7 antibody or IgG on days 21–25 and liver specimens collected on day 42 ( n = 5 mice in each group). ( B ) Representative micrographs of Masson trichrome stained liver tissue sections. Quantification of collagen staining in the liver. Magnification: ×40. ( C ) Representative images of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in the liver (Arrows indicate biliary epithelial cells). ( D ) Serum level of sE-cadherin in mice ( n = 5 mice in each group). ( E ) Representative images of immunostaining β-catenin in cholangiocytes of mice. ( F ) Serum level of MMP7 in mice ( n = 5 mice in each group). ( G ) Hepatic mRNA expression of MMP7 in mice ( n = 5 mice in each group). Magnification: left panel : ×100; right panel : ×400. Data are presented as mean ± SD.

    Journal: International Journal of Molecular Sciences

    Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

    doi: 10.3390/ijms27052209

    Figure Lengend Snippet: MMP7 blockade attenuated liver fibrosis and EMT procession in chronic BA mice. ( A ) Schematic representation of chronic BA mice injected with anti-MMP7 antibody or IgG on days 21–25 and liver specimens collected on day 42 ( n = 5 mice in each group). ( B ) Representative micrographs of Masson trichrome stained liver tissue sections. Quantification of collagen staining in the liver. Magnification: ×40. ( C ) Representative images of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in the liver (Arrows indicate biliary epithelial cells). ( D ) Serum level of sE-cadherin in mice ( n = 5 mice in each group). ( E ) Representative images of immunostaining β-catenin in cholangiocytes of mice. ( F ) Serum level of MMP7 in mice ( n = 5 mice in each group). ( G ) Hepatic mRNA expression of MMP7 in mice ( n = 5 mice in each group). Magnification: left panel : ×100; right panel : ×400. Data are presented as mean ± SD.

    Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

    Techniques: Injection, Staining, Immunostaining, Expressing